PCR and Southern analysis showed that the T-DNA was integrated into the genome. Among of the examined transformants contained a single copy, and was stable through mitotic cell division. 通过对转化子的PCR检测和Southern杂交分析表明,TDNA已整合进毛壳菌基因组中,而且在所检测的转化子中都是以单拷贝的形式整合,转化子都能够稳定遗传。
Considering the recovery incubation time may affect the integration copy number, we examined the impact of different recovery incubation time on random integration copy numbers. 考虑到恢复培养时间的长短可能会影响整合的最大拷贝数,我们检测了不同恢复培养时间的影响。